The present study is concerned with the phenomenon of topicalization in Pashto. It analyzed the information status of the referents in a sentence and their grammatical functions. The study is another contribution after the work of Roberts (2000) and Rahman (2014). The study was descriptive in nature and it found out the relationship between subject and topic in a sentence. The grammatical function of the subject and the topic were found out coincidentally occurring with the topic of the sentence. Both the subject and topic were found out commonly appearing at the initial position of the sentence. If the topic was any other constituent than the subject, then topicalization happened. The topicalized element is moved to the second or initial position of the sentence. Word order is determined by the information status of the referents where the topic comes first followed by the focused constituents, if not overruled by intonational prominence.